Cell Culture Bioreactors
ثبت نشده
چکیده
Cell Culture Bioreactors Basic Types of Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Stirred Tank (Well Mixed) vs. Tubular Reactor (Plug Flow) . . . . . . . . . . . . . . . . . 3 Segregated Bioreactors (Dead Zone Present) Compartmentalized Bioreactors . . 4 Implication When Growth or Reaction Occurs in the Reactor . . . . . . . . . . . . . . . 4 Homogenous Reactor vs. Heterogeneous Reactor . . . . . . . . . . . . . . . . . . . . . . . . . 4 Operating Mode of Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 Batch and Continuous Processes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 The Operating Mode of Reactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Batch Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Fedbatch Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Material Balance on Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Material Balance Equation for Reactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Tissue culture and disposable cell culture systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Tissue Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Disposable Culture Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Multiple Plate Culture System (Cell Cube and Cell Factory) . . . . . . . . . . . . . . . . 2 Cell Support Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Suspension Culture vs Adherent Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Microcarriers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Cell Aggregates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Microsphere Induce Cell Aggregates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Agarose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Microencapsulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Cell Culture Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Simple Stirred Tank Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Airlift Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Membrane Stirred Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Spin Filter Stirred Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Vibromixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Fluidized Bed Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 Membrane Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
منابع مشابه
Highly efficient inoculum propagation in perfusion culture using WAVE BioreactorTM systems
Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...
متن کاملHighly efficient inoculum propagation in perfusion culture using WAVE Bioreactor™ systems
Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...
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The gas sparging of culture broth causes damage to suspended animal cells. However, despite this, sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen, especially on a large scale. This article examines the underlying mechanisms involved in bubble-associated cell damage and the methods available for controlling such damage.
متن کاملTwo new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor.
The present article describes two novel flexible plastic-based disposable bioreactors. The first one, the WU bioreactor, is based on the principle of a wave and undertow mechanism that provides agitation while offering convenient mixing and aeration to the plant cell culture contained within the bioreactor. The second one is a high aspect ratio bubble column bioreactor, where agitation and aera...
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